Spawning is mixing spawn into cooled substrate in a clean room. Oyster uses 2 to 3 percent of wet weight in polythene bags of 40 to 45 by 30 to 35 cm with 10 to 12 small holes, according to the folder.
Colonisation, or spawn run, is the wait while the bag turns white. Follow the species-specific bag and incubation protocol. The crop respires and releases heat; room ventilation and monitoring remain necessary. Oyster takes 15 to 25 days and DMR says never open bags before 16 to 18 days. Button takes 12 to 14 days, then a 3 to 4 cm casing layer of soil is laid on top and a further week of case run follows. Milky takes 15 to 20 days, then casing and 10 to 12 days of case run.